PoplarV1, Main, Exploration, bibRecord, 000876

Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.

Identifieur interne : 000876 ( Main/Exploration ); précédent : 000875; suivant : 000877

Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.

Auteurs : Jeffrey Butler [Australie] ; Deborah Middleton [Australie] ; Jessica Haining [Australie] ; Rachel Layton [Australie] ; Steven Rockman [Australie] ; Lorena E. Brown [Australie] ; Sandra Sapats [Australie]

Source :

Viruses [ 1999-4915 ] ; 2019.

RBID : pubmed:31590265

Descripteurs français

KwdFr :
- Animaux (MeSH), Appareil respiratoire (MeSH), Cellules Vero (MeSH), Foie (anatomopathologie), Furets (virologie), Grippe chez les oiseaux (anatomopathologie), Grippe chez les oiseaux (métabolisme), Grippe chez les oiseaux (virologie), Grippe humaine (virologie), Humains (MeSH), Infections à Orthomyxoviridae (virologie), Modèles animaux de maladie humaine (MeSH), Mutagenèse dirigée (MeSH), Polymorphisme de nucléotide simple (MeSH), Poulets (MeSH), Réplication virale (MeSH), Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (pathogénicité), Sous-type H5N1 du virus de la grippe A (physiologie), Substitution d'acide aminé (MeSH), Virulence (MeSH).
MESH :
- anatomopathologie : Foie, Grippe chez les oiseaux.
- génétique : Sous-type H5N1 du virus de la grippe A.
- métabolisme : Grippe chez les oiseaux.
- pathogénicité : Sous-type H5N1 du virus de la grippe A.
- physiologie : Sous-type H5N1 du virus de la grippe A.
- virologie : Furets, Grippe chez les oiseaux, Grippe humaine, Infections à Orthomyxoviridae.
- Animaux, Appareil respiratoire, Cellules Vero, Humains, Modèles animaux de maladie humaine, Mutagenèse dirigée, Polymorphisme de nucléotide simple, Poulets, Réplication virale, Substitution d'acide aminé, Virulence.

English descriptors

KwdEn :
- Amino Acid Substitution (MeSH), Animals (MeSH), Chickens (MeSH), Chlorocebus aethiops (MeSH), Disease Models, Animal (MeSH), Ferrets (virology), Humans (MeSH), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (pathogenicity), Influenza A Virus, H5N1 Subtype (physiology), Influenza in Birds (metabolism), Influenza in Birds (pathology), Influenza in Birds (virology), Influenza, Human (virology), Liver (pathology), Mutagenesis, Site-Directed (MeSH), Orthomyxoviridae Infections (virology), Polymorphism, Single Nucleotide (MeSH), Respiratory System (MeSH), Vero Cells (MeSH), Virulence (MeSH), Virus Replication (MeSH).
MESH :
- genetics : Influenza A Virus, H5N1 Subtype.
- metabolism : Influenza in Birds.
- pathogenicity : Influenza A Virus, H5N1 Subtype.
- pathology : Influenza in Birds, Liver.
- physiology : Influenza A Virus, H5N1 Subtype.
- virology : Ferrets, Influenza in Birds, Influenza, Human, Orthomyxoviridae Infections.
- Amino Acid Substitution, Animals, Chickens, Chlorocebus aethiops, Disease Models, Animal, Humans, Mutagenesis, Site-Directed, Polymorphism, Single Nucleotide, Respiratory System, Vero Cells, Virulence, Virus Replication.

Abstract

Circulating avian influenza viruses pose a significant threat, with human infections occurring infrequently but with potentially severe consequences. To examine the dynamics and locale of the adaptation process of avian influenza viruses when introduced to a mammalian host, we infected ferrets with H5N1 viruses. As expected, all ferrets infected with the human H5N1 isolate A/Vietnam/1203/2004 showed severe disease and virus replication outside the respiratory tract in multiple organs including the brain. In contrast infection of ferrets with the avian H5N1 virus A/Chicken/Laos/Xaythiani26/2006 showed a different collective pattern of infection; many ferrets developed and cleared a mild respiratory infection but a subset (25-50%), showed extended replication in the upper respiratory tract and developed infection in distal sites. Virus from these severely infected ferrets was commonly found in tissues that included liver and small intestine. In most instances the virus had acquired the common virulence substitution PB2 E627K but, in one case, a previously unidentified combination of two amino acid substitutions at PB2 S489P and NP V408I, which enhanced polymerase activity, was found. We noted that virus with high pathogenicity adaptations could be dominant in an extra-respiratory site without being equally represented in the nasal wash. Further ferret passage of these mutated viruses resulted in high pathogenicity in all ferrets. These findings illustrate the remarkable ability of avian influenza viruses that avoid clearance in the respiratory tract, to mutate towards a high pathogenicity phenotype during just a single passage in ferrets and also indicate a window of less than 5 days in which treatment may curtail systemic infection.

DOI: 10.3390/v11100915
PubMed: 31590265
PubMed Central: PMC6832663

Affiliations:

Australie

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000676
to stream Main, to step Curation: 000676

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.</title>
<author><name sortKey="Butler, Jeffrey" sort="Butler, Jeffrey" uniqKey="Butler J" first="Jeffrey" last="Butler">Jeffrey Butler</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Middleton, Deborah" sort="Middleton, Deborah" uniqKey="Middleton D" first="Deborah" last="Middleton">Deborah Middleton</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Haining, Jessica" sort="Haining, Jessica" uniqKey="Haining J" first="Jessica" last="Haining">Jessica Haining</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Layton, Rachel" sort="Layton, Rachel" uniqKey="Layton R" first="Rachel" last="Layton">Rachel Layton</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E" last="Brown">Lorena E. Brown</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia. lorena@unimelb.edu.au.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sapats, Sandra" sort="Sapats, Sandra" uniqKey="Sapats S" first="Sandra" last="Sapats">Sandra Sapats</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:31590265</idno>
<idno type="pmid">31590265</idno>
<idno type="doi">10.3390/v11100915</idno>
<idno type="pmc">PMC6832663</idno>
<idno type="wicri:Area/Main/Corpus">000676</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000676</idno>
<idno type="wicri:Area/Main/Curation">000676</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000676</idno>
<idno type="wicri:Area/Main/Exploration">000676</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.</title>
<author><name sortKey="Butler, Jeffrey" sort="Butler, Jeffrey" uniqKey="Butler J" first="Jeffrey" last="Butler">Jeffrey Butler</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Middleton, Deborah" sort="Middleton, Deborah" uniqKey="Middleton D" first="Deborah" last="Middleton">Deborah Middleton</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Haining, Jessica" sort="Haining, Jessica" uniqKey="Haining J" first="Jessica" last="Haining">Jessica Haining</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Layton, Rachel" sort="Layton, Rachel" uniqKey="Layton R" first="Rachel" last="Layton">Rachel Layton</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E" last="Brown">Lorena E. Brown</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia. lorena@unimelb.edu.au.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sapats, Sandra" sort="Sapats, Sandra" uniqKey="Sapats S" first="Sandra" last="Sapats">Sandra Sapats</name>
<affiliation wicri:level="1"><nlm:affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Viruses</title>
<idno type="eISSN">1999-4915</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Substitution (MeSH)</term>
<term>Animals (MeSH)</term>
<term>Chickens (MeSH)</term>
<term>Chlorocebus aethiops (MeSH)</term>
<term>Disease Models, Animal (MeSH)</term>
<term>Ferrets (virology)</term>
<term>Humans (MeSH)</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N1 Subtype (pathogenicity)</term>
<term>Influenza A Virus, H5N1 Subtype (physiology)</term>
<term>Influenza in Birds (metabolism)</term>
<term>Influenza in Birds (pathology)</term>
<term>Influenza in Birds (virology)</term>
<term>Influenza, Human (virology)</term>
<term>Liver (pathology)</term>
<term>Mutagenesis, Site-Directed (MeSH)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Polymorphism, Single Nucleotide (MeSH)</term>
<term>Respiratory System (MeSH)</term>
<term>Vero Cells (MeSH)</term>
<term>Virulence (MeSH)</term>
<term>Virus Replication (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Appareil respiratoire (MeSH)</term>
<term>Cellules Vero (MeSH)</term>
<term>Foie (anatomopathologie)</term>
<term>Furets (virologie)</term>
<term>Grippe chez les oiseaux (anatomopathologie)</term>
<term>Grippe chez les oiseaux (métabolisme)</term>
<term>Grippe chez les oiseaux (virologie)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains (MeSH)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Modèles animaux de maladie humaine (MeSH)</term>
<term>Mutagenèse dirigée (MeSH)</term>
<term>Polymorphisme de nucléotide simple (MeSH)</term>
<term>Poulets (MeSH)</term>
<term>Réplication virale (MeSH)</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N1 du virus de la grippe A (pathogénicité)</term>
<term>Sous-type H5N1 du virus de la grippe A (physiologie)</term>
<term>Substitution d'acide aminé (MeSH)</term>
<term>Virulence (MeSH)</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Foie</term>
<term>Grippe chez les oiseaux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza in Birds</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Grippe chez les oiseaux</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Influenza in Birds</term>
<term>Liver</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Furets</term>
<term>Grippe chez les oiseaux</term>
<term>Grippe humaine</term>
<term>Infections à Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Ferrets</term>
<term>Influenza in Birds</term>
<term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Substitution</term>
<term>Animals</term>
<term>Chickens</term>
<term>Chlorocebus aethiops</term>
<term>Disease Models, Animal</term>
<term>Humans</term>
<term>Mutagenesis, Site-Directed</term>
<term>Polymorphism, Single Nucleotide</term>
<term>Respiratory System</term>
<term>Vero Cells</term>
<term>Virulence</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Appareil respiratoire</term>
<term>Cellules Vero</term>
<term>Humains</term>
<term>Modèles animaux de maladie humaine</term>
<term>Mutagenèse dirigée</term>
<term>Polymorphisme de nucléotide simple</term>
<term>Poulets</term>
<term>Réplication virale</term>
<term>Substitution d'acide aminé</term>
<term>Virulence</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Circulating avian influenza viruses pose a significant threat, with human infections occurring infrequently but with potentially severe consequences. To examine the dynamics and locale of the adaptation process of avian influenza viruses when introduced to a mammalian host, we infected ferrets with H5N1 viruses. As expected, all ferrets infected with the human H5N1 isolate A/Vietnam/1203/2004 showed severe disease and virus replication outside the respiratory tract in multiple organs including the brain. In contrast infection of ferrets with the avian H5N1 virus A/Chicken/Laos/Xaythiani26/2006 showed a different collective pattern of infection; many ferrets developed and cleared a mild respiratory infection but a subset (25-50%), showed extended replication in the upper respiratory tract and developed infection in distal sites. Virus from these severely infected ferrets was commonly found in tissues that included liver and small intestine. In most instances the virus had acquired the common virulence substitution PB2 E627K but, in one case, a previously unidentified combination of two amino acid substitutions at PB2 S489P and NP V408I, which enhanced polymerase activity, was found. We noted that virus with high pathogenicity adaptations could be dominant in an extra-respiratory site without being equally represented in the nasal wash. Further ferret passage of these mutated viruses resulted in high pathogenicity in all ferrets. These findings illustrate the remarkable ability of avian influenza viruses that avoid clearance in the respiratory tract, to mutate towards a high pathogenicity phenotype during just a single passage in ferrets and also indicate a window of less than 5 days in which treatment may curtail systemic infection.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31590265</PMID>
<DateCompleted><Year>2020</Year>
<Month>09</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>10</Issue>
<PubDate><Year>2019</Year>
<Month>10</Month>
<Day>04</Day>
</PubDate>
</JournalIssue>
<Title>Viruses</Title>
<ISOAbbreviation>Viruses</ISOAbbreviation>
</Journal>
<ArticleTitle>Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">E915</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/v11100915</ELocationID>
<Abstract><AbstractText>Circulating avian influenza viruses pose a significant threat, with human infections occurring infrequently but with potentially severe consequences. To examine the dynamics and locale of the adaptation process of avian influenza viruses when introduced to a mammalian host, we infected ferrets with H5N1 viruses. As expected, all ferrets infected with the human H5N1 isolate A/Vietnam/1203/2004 showed severe disease and virus replication outside the respiratory tract in multiple organs including the brain. In contrast infection of ferrets with the avian H5N1 virus A/Chicken/Laos/Xaythiani26/2006 showed a different collective pattern of infection; many ferrets developed and cleared a mild respiratory infection but a subset (25-50%), showed extended replication in the upper respiratory tract and developed infection in distal sites. Virus from these severely infected ferrets was commonly found in tissues that included liver and small intestine. In most instances the virus had acquired the common virulence substitution PB2 E627K but, in one case, a previously unidentified combination of two amino acid substitutions at PB2 S489P and NP V408I, which enhanced polymerase activity, was found. We noted that virus with high pathogenicity adaptations could be dominant in an extra-respiratory site without being equally represented in the nasal wash. Further ferret passage of these mutated viruses resulted in high pathogenicity in all ferrets. These findings illustrate the remarkable ability of avian influenza viruses that avoid clearance in the respiratory tract, to mutate towards a high pathogenicity phenotype during just a single passage in ferrets and also indicate a window of less than 5 days in which treatment may curtail systemic infection.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Butler</LastName>
<ForeName>Jeffrey</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Middleton</LastName>
<ForeName>Deborah</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Haining</LastName>
<ForeName>Jessica</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Layton</LastName>
<ForeName>Rachel</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rockman</LastName>
<ForeName>Steven</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Brown</LastName>
<ForeName>Lorena E</ForeName>
<Initials>LE</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Melbourne 3000, Victoria, Australia. lorena@unimelb.edu.au.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sapats</LastName>
<ForeName>Sandra</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>The Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory (CSIRO-AAHL), Geelong 3219, Victoria, Australia.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>10</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Viruses</MedlineTA>
<NlmUniqueID>101509722</NlmUniqueID>
<ISSNLinking>1999-4915</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005289" MajorTopicYN="N">Ferrets</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008099" MajorTopicYN="N">Liver</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009976" MajorTopicYN="N">Orthomyxoviridae Infections</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020641" MajorTopicYN="N">Polymorphism, Single Nucleotide</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012137" MajorTopicYN="N">Respiratory System</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">H5N1 influenza virus</Keyword>
<Keyword MajorTopicYN="Y">avian influenza</Keyword>
<Keyword MajorTopicYN="Y">ferret</Keyword>
<Keyword MajorTopicYN="Y">mammalian adaptation</Keyword>
<Keyword MajorTopicYN="Y">systemic infection</Keyword>
</KeywordList>
<CoiStatement>The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>08</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>09</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>09</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>10</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>10</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>9</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31590265</ArticleId>
<ArticleId IdType="pii">v11100915</ArticleId>
<ArticleId IdType="doi">10.3390/v11100915</ArticleId>
<ArticleId IdType="pmc">PMC6832663</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Sci Rep. 2018 Aug 30;8(1):13066</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30166556</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 May 16;368(20):1888-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23577628</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2011 Jan 18;2:156</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21245837</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2000 May 23;97(11):6108-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10801978</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2006 Jun;80(12):5976-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16731936</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci China Life Sci. 2013 Jun;56(6):485-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23657795</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2016 Jun 10;90(13):5928-5938</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27076644</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013 Apr 11;18(15):20453</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23594575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Oct;87(19):10884-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23864613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Microbiol. 2008;62:403-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18785841</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2010 Sep;87(3):361-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20603155</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Jul;87(13):7200-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23616660</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013 Apr 18;18(16):20460</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23611030</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2016 Apr 20;12(4):e1005583</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27097026</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2015 Jun 17;6:7491</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26082035</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2012 May 02;486(7403):420-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22722205</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2006 Oct;12(10):1203-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16964257</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2008 Jan 17;358(3):261-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18199865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Jun 22;336(6088):1534-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22723413</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Oct;85(20):10691-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21849466</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2009 Oct;53(10):4433-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19651908</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1993 Apr;67(4):1761-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8445709</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2014 Nov 20;5:5509</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25409547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2007 Oct 5;3(10):1374-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17922570</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2008 Feb 8;4(2):e11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18248089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2007 Jun 5;362(2):271-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17270230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2005 Feb 17;352(7):686-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15716562</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21312-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19995968</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2005 Jul 14;436(7048):191-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16007072</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Sep;79(18):11788-800</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16140756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2012 Jan;8(1):e1002488</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22275867</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Aug 19;309(5738):1206</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16000410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2007 Jul 20;364(1):28-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17408714</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2001 Jun;75(11):5398-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11333924</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2005 Sep 29;353(13):1374-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16192482</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2019 May 13;:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31081750</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Microbes Infect. 2014 Feb;3(2):e9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26038511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Feb;83(3):1320-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19019950</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18590-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16339318</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Aug;88(16):8735-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24899203</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2000 May;81(Pt 5):1283-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10769071</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2004 Mar 15;320(2):258-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15016548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Nov;88(22):13436-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25210184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2005 Jan 27;352(4):333-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15668219</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Australie</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Butler, Jeffrey" sort="Butler, Jeffrey" uniqKey="Butler J" first="Jeffrey" last="Butler">Jeffrey Butler</name>
</noRegion>
<name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E" last="Brown">Lorena E. Brown</name>
<name sortKey="Butler, Jeffrey" sort="Butler, Jeffrey" uniqKey="Butler J" first="Jeffrey" last="Butler">Jeffrey Butler</name>
<name sortKey="Haining, Jessica" sort="Haining, Jessica" uniqKey="Haining J" first="Jessica" last="Haining">Jessica Haining</name>
<name sortKey="Layton, Rachel" sort="Layton, Rachel" uniqKey="Layton R" first="Rachel" last="Layton">Rachel Layton</name>
<name sortKey="Middleton, Deborah" sort="Middleton, Deborah" uniqKey="Middleton D" first="Deborah" last="Middleton">Deborah Middleton</name>
<name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name>
<name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name>
<name sortKey="Sapats, Sandra" sort="Sapats, Sandra" uniqKey="Sapats S" first="Sandra" last="Sapats">Sandra Sapats</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000876 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000876 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:31590265
   |texte=   Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:31590265" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.

Insights into the Acquisition of Virulence of Avian Influenza Viruses during a Single Passage in Ferrets.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki